Mouse Haptoglobin ELISA Kit PicoKine®

Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2678 2633 2766 2754 2707 54.78 2% Sample 2 12849 14795 13542 15077 14065 909.56 6.4% Sample 3 42168 46553 47581 44731 45258 2055.43 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2678 2633 2766 2754 2707 54.78 2% Sample 1 2678 2633 2766 2754 2707 54.78 2% Sample 2 12849 14795 13542 15077 14065 909.56 6.4% Sample 2 12849 14795 13542 15077 14065 909.56 6.4% Sample 3 42168 46553 47581 44731 45258 2055.43 4.5% Sample 3 42168 46553 47581 44731 45258 2055.43 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 CV (%) 7.5% 7.6% 4.7% 7.8% 7.8% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 CV (%) 7.5% 7.6% 4.7% 7.8% 7.8% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Mean (pg/ml) 2678 12849 42168 2657 13883 41875 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 Standard deviation 200.85 976.52 1981.89 207.24 1082.87 2093.75 CV (%) 7.5% 7.6% 4.7% 7.8% 7.8% 5% CV (%) 7.5% 7.6% 4.7% 7.8% 7.8% 5%

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity) .By similarity Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.015 0.047 0.079 0.148 0.309 0.646 1.322 2.229 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.015 0.047 0.079 0.148 0.309 0.646 1.322 2.229 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.015 0.047 0.079 0.148 0.309 0.646 1.322 2.229 O.D. 0.015 0.047 0.079 0.148 0.309 0.646 1.322 2.229

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.