Human MADCAM-1 ELISA Kit PicoKine®

MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4/beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 73 78 81 76 3.67 4.8% Sample 2 241 227 222 222 228 7.77 3.4% Sample 3 1030 1091 1082 1036 1059 27.02 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 73 78 81 76 3.67 4.8% Sample 1 72 73 78 81 76 3.67 4.8% Sample 2 241 227 222 222 228 7.77 3.4% Sample 2 241 227 222 222 228 7.77 3.4% Sample 3 1030 1091 1082 1036 1059 27.02 2.5% Sample 3 1030 1091 1082 1036 1059 27.02 2.5%

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

Angiogenesis, Cardiovascular, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, Immunology, Innate Immunity, Integrins, Macrophage/Inflammation, Neuroscience, Receptors, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 241 1030 77 233 996 Standard deviation 4.96 16.5%6 62.83 6.1%2 16.07 62.74 CV (%) 6.9% 6.5% 6.1% 7.7% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 241 1030 77 233 996 Standard deviation 4.96 16.5%6 62.83 6.1%2 16.07 62.74 CV (%) 6.9% 6.5% 6.1% 7.7% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 72 241 1030 77 233 996 Mean (pg/ml) 72 241 1030 77 233 996 Standard deviation 4.96 16.5%6 62.83 6.1%2 16.07 62.74 Standard deviation 4.96 16.5%6 62.83 6.1%2 16.07 62.74 CV (%) 6.9% 6.5% 6.1% 7.7% 6.9% 6.3% CV (%) 6.9% 6.5% 6.1% 7.7% 6.9% 6.3%

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.127 0.212 0.373 0.642 1.085 1.709 2.383 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.127 0.212 0.373 0.642 1.085 1.709 2.383 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.127 0.212 0.373 0.642 1.085 1.709 2.383 O.D. 0.03 0.127 0.212 0.373 0.642 1.085 1.709 2.383

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.