Human Uromodulin/umod ELISA Kit PicoKine®

The Tamm–Horsfall glycoprotein (THP), also known as uromodulin, is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN) . Alternative splicing of this gene results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 216 203 201 208 6.79 3.2% Sample 2 1090 1078 1129 1138 1108 25.31 2.2% Sample 3 5159 5083 5851 5299 5348 300.56 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 215 216 203 201 208 6.79 3.2% Sample 1 215 216 203 201 208 6.79 3.2% Sample 2 1090 1078 1129 1138 1108 25.31 2.2% Sample 2 1090 1078 1129 1138 1108 25.31 2.2% Sample 3 5159 5083 5851 5299 5348 300.56 5.6% Sample 3 5159 5083 5851 5299 5348 300.56 5.6%

Expressed in the tubular cells of the kidney. Most abundant protein in normal urine (at protein level) . Synthesized exclusively in the kidney. Expressed exclusively by epithelial cells of the thick ascending limb of Henle's loop (TALH) and of distal convoluted tubule lumen.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1090 5159 226 982 5437 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 CV (%) 6.2% 5.3% 4.1% 7.4% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 215 1090 5159 226 982 5437 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 CV (%) 6.2% 5.3% 4.1% 7.4% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 215 1090 5159 226 982 5437 Mean (pg/ml) 215 1090 5159 226 982 5437 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 Standard deviation 13.33 57.77 211.51 16.72 62.84 293.59 CV (%) 6.2% 5.3% 4.1% 7.4% 6.4% 5.4% CV (%) 6.2% 5.3% 4.1% 7.4% 6.4% 5.4%

Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable) . May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF (PubMed:3498215) . Facilitates neutrophil migration across renal epithelia (PubMed:20798515) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.075 0.138 0.242 0.45 0.81 1.338 1.916 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.075 0.138 0.242 0.45 0.81 1.338 1.916 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.075 0.138 0.242 0.45 0.81 1.338 1.916 O.D. 0.024 0.075 0.138 0.242 0.45 0.81 1.338 1.916

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.