Human Klotho ELISA Kit PicoKine®

Klotho (KL) is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss and alcohol consumption.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 313 332 326 319 9.65 3% Sample 2 1224 1059 1107 1175 1141 63.09 5.5% Sample 3 5567 6048 6003 5069 5671 395.46 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 308 313 332 326 319 9.65 3% Sample 1 308 313 332 326 319 9.65 3% Sample 2 1224 1059 1107 1175 1141 63.09 5.5% Sample 2 1224 1059 1107 1175 1141 63.09 5.5% Sample 3 5567 6048 6003 5069 5671 395.46 6.9% Sample 3 5567 6048 6003 5069 5671 395.46 6.9%

Present in cortical renal tubules (at protein level) . Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1224 5567 337 1220 5449 Standard deviation 15.4 79.56 239.38 20.55 102.48 321.49 CV (%) 5% 6.5% 4.3% 6.1% 8.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 308 1224 5567 337 1220 5449 Standard deviation 15.4 79.56 239.38 20.55 102.48 321.49 CV (%) 5% 6.5% 4.3% 6.1% 8.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 308 1224 5567 337 1220 5449 Mean (pg/ml) 308 1224 5567 337 1220 5449 Standard deviation 15.4 79.56 239.38 20.55 102.48 321.49 Standard deviation 15.4 79.56 239.38 20.55 102.48 321.49 CV (%) 5% 6.5% 4.3% 6.1% 8.4% 5.9% CV (%) 5% 6.5% 4.3% 6.1% 8.4% 5.9%

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity) . Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.138 0.208 0.338 0.598 1.039 1.716 2.616 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.138 0.208 0.338 0.598 1.039 1.716 2.616 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.138 0.208 0.338 0.598 1.039 1.716 2.616 O.D. 0.065 0.138 0.208 0.338 0.598 1.039 1.716 2.616

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.