Human Cathepsin H ELISA Kit PicoKine®

Human Cathepsin H ELISA Kit PicoKine® (96 Tests) . Quantitate Human CTSH in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin H is a protein that in humans is encoded by the CTSH gene. The protein encoded by this gene gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 372 315 318 335 335 22.68 6.7% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 3 4095 4519 4622 4221 4364 214.12 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 372 315 318 335 335 22.68 6.7% Sample 1 372 315 318 335 335 22.68 6.7% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 2 987 987 1084 1153 1052 70.12 6.6% Sample 3 4095 4519 4622 4221 4364 214.12 4.9% Sample 3 4095 4519 4622 4221 4364 214.12 4.9%

Synonyms

Pro-cathepsin H; Cathepsin H mini chain; Cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; CTSH; CPSB

Gene Name

CTSH

Gene ID

1512

UniProt

P09668

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: A23-V335

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Alzheimer's Disease, Cell Adhesion Proteins, Membrane Proteins, Neurodegenerative Disease, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 372 987 40395 395 1022 3714 Mean (pg/ml) 372 987 40395 395 1022 3714 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 Standard deviation 16.74 68.1 1622.919 22.91 80.73 185.7 CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5% CV (%) 4.5% 6.9% 4.1% 5.8% 7.9% 5%

Sensitivity

<10 pg/mL

Function

Important for the overall degradation of proteins in lysosomes.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Cathepsin H

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin H

Subcellular Location

Lysosome.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764 O.D. 0.043 0.077 0.109 0.167 0.283 0.515 0.944 1.764

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.