Human TREM2 ELISA Kit PicoKine®

Triggering receptor expressed on myeloid cells 2 (TRM2) encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) . Alternative splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 67 69 70 69 1.8 2.6% Sample 2 232 244 222 251 237 11.12 4.6% Sample 3 780 887 891 824 845 46.21 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 67 69 70 69 1.8 2.6% Sample 1 72 67 69 70 69 1.8 2.6% Sample 2 232 244 222 251 237 11.12 4.6% Sample 2 232 244 222 251 237 11.12 4.6% Sample 3 780 887 891 824 845 46.21 5.4% Sample 3 780 887 891 824 845 46.21 5.4%

Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 232 780 74 222 829 Standard deviation 2.95 9.74 37.44 3.1 10.65 55.54 CV (%) 4.1% 4.2% 4.8% 4.2% 4.8% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 232 780 74 222 829 Standard deviation 2.95 9.74 37.44 3.1 10.65 55.54 CV (%) 4.1% 4.2% 4.8% 4.2% 4.8% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 72 232 780 74 222 829 Mean (pg/ml) 72 232 780 74 222 829 Standard deviation 2.95 9.74 37.44 3.1 10.65 55.54 Standard deviation 2.95 9.74 37.44 3.1 10.65 55.54 CV (%) 4.1% 4.2% 4.8% 4.2% 4.8% 6.7% CV (%) 4.1% 4.2% 4.8% 4.2% 4.8% 6.7%

Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.079 0.185 0.264 0.407 0.634 1.056 1.69 2.674 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.079 0.185 0.264 0.407 0.634 1.056 1.69 2.674 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.079 0.185 0.264 0.407 0.634 1.056 1.69 2.674 O.D. 0.079 0.185 0.264 0.407 0.634 1.056 1.69 2.674

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.