Human TRKC ELISA Kit PicoKine®

Human TRKC ELISA Kit PicoKine® (96 Tests) . Quantitate Human NTRK3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tropomyosin receptor kinase C? (TrkC), ?also known as?NT-3 growth factor receptor, ?neurotrophic tyrosine kinase receptor type 3, or?TrkC tyrosine kinase?is a?protein?that in humans is encoded by the?NTRK3?gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.?

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 342 297 342 327 18.41 5.6% Sample 2 1741 1582 1616 1731 1667 69.63 4.1% Sample 3 4941 4243 4806 4622 4653 262.39 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 342 297 342 327 18.41 5.6% Sample 1 330 342 297 342 327 18.41 5.6% Sample 2 1741 1582 1616 1731 1667 69.63 4.1% Sample 2 1741 1582 1616 1731 1667 69.63 4.1% Sample 3 4941 4243 4806 4622 4653 262.39 5.6% Sample 3 4941 4243 4806 4622 4653 262.39 5.6%

Synonyms

NT-3 growth factor receptor; GP145-TrkC; Trk-C; Neurotrophic tyrosine kinase receptor type 3; TrkC tyrosine kinase; NTRK3; TRKC

Gene Name

NTRK3

Gene ID

4916

UniProt

Q16288

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C32-A428

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Applications

ELISA

Field of Research

Cancer, Growth and Development, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1741 4941 335 1753 4967 Standard deviation 18.15 85.3 390.33 22.78 92.9 466.89 CV (%) 5.5% 4.9% 7.9% 6.8% 5.3% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1741 4941 335 1753 4967 Standard deviation 18.15 85.3 390.33 22.78 92.9 466.89 CV (%) 5.5% 4.9% 7.9% 6.8% 5.3% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 330 1741 4941 335 1753 4967 Mean (pg/ml) 330 1741 4941 335 1753 4967 Standard deviation 18.15 85.3 390.33 22.78 92.9 466.89 Standard deviation 18.15 85.3 390.33 22.78 92.9 466.89 CV (%) 5.5% 4.9% 7.9% 6.8% 5.3% 9.4% CV (%) 5.5% 4.9% 7.9% 6.8% 5.3% 9.4%

Sensitivity

<10 pg/mL

Function

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TRKC

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neurotrophic receptor tyrosine kinase 3

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.225 0.323 0.462 0.792 1.291 1.97 2.548 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.225 0.323 0.462 0.792 1.291 1.97 2.548 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.225 0.323 0.462 0.792 1.291 1.97 2.548 O.D. 0.084 0.225 0.323 0.462 0.792 1.291 1.97 2.548

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.