Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

Alpha-1-antitrypsin?or?α1-antitrypsin? (A1AT, ?A1A, or?AAT) is a?protein?belonging to the?serpin?superfamily. It is encoded in humans by the?SERPINA1?gene.?The?gene?is located on the long arm of the fourteenth?chromosome? (14q32.1) .The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all?serine protease inhibitors, A1AT has a characteristic?secondary structure?of?beta sheets?and?alpha helices.?Mutations?in these areas can lead to non-functional proteins that can?polymerise?and accumulate in the?liver (infantile hepatic cirrhosis) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2451 2297 2432 2102 2320 139.42 6% Sample 2 15117 15111 14581 14540 14837 277.13 1.8% Sample 3 38332 38692 42108 38880 39503 1516.83 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2451 2297 2432 2102 2320 139.42 6% Sample 1 2451 2297 2432 2102 2320 139.42 6% Sample 2 15117 15111 14581 14540 14837 277.13 1.8% Sample 2 15117 15111 14581 14540 14837 277.13 1.8% Sample 3 38332 38692 42108 38880 39503 1516.83 3.8% Sample 3 38332 38692 42108 38880 39503 1516.83 3.8%

Intracellular, Kinases, Metabolism, Metabolism Processes, Pathways and Processes, Protease Inhibitors, Proteolysis/Ubiquitin, Response To Hypoxia, Serum Proteins

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2451 15117 38332 2323 15142 40979 Standard deviation 144.6 1179.12 2529.91 157.96 1423.34 3114.4 CV (%) 6.6%% 7.8% 6.6% 6.8% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2451 15117 38332 2323 15142 40979 Standard deviation 144.6 1179.12 2529.91 157.96 1423.34 3114.4 CV (%) 6.6%% 7.8% 6.6% 6.8% 9.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2451 15117 38332 2323 15142 40979 Mean (pg/ml) 2451 15117 38332 2323 15142 40979 Standard deviation 144.6 1179.12 2529.91 157.96 1423.34 3114.4 Standard deviation 144.6 1179.12 2529.91 157.96 1423.34 3114.4 CV (%) 6.6%% 7.8% 6.6% 6.8% 9.4% 7.6% CV (%) 6.6%% 7.8% 6.6% 6.8% 9.4% 7.6%

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.053 0.113 0.171 0.318 0.525 0.96 1.489 2.089 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.053 0.113 0.171 0.318 0.525 0.96 1.489 2.089 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.053 0.113 0.171 0.318 0.525 0.96 1.489 2.089 O.D. 0.053 0.113 0.171 0.318 0.525 0.96 1.489 2.089

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.