Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®

Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 456 596 343 482 469 35.71 7.6% Sample 2 1624 977 1230 767 1150 82.99 7.2% Sample 3 9389 6426 8298 6358 7618 509.63 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 456 596 343 482 469 35.71 7.6% Sample 1 456 596 343 482 469 35.71 7.6% Sample 2 1624 977 1230 767 1150 82.99 7.2% Sample 2 1624 977 1230 767 1150 82.99 7.2% Sample 3 9389 6426 8298 6358 7618 509.63 6.7% Sample 3 9389 6426 8298 6358 7618 509.63 6.7%

Detected in serum and cerebrospinal fluid (at protein level) . Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 456 1624 9289 529 1947 9298 Standard deviation 34.34 55.87 599.02 33.49 38.94 304.04 CV (%) 7.5% 3.4% 6.4% 6.3% 2% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 456 1624 9289 529 1947 9298 Standard deviation 34.34 55.87 599.02 33.49 38.94 304.04 CV (%) 7.5% 3.4% 6.4% 6.3% 2% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 456 1624 9289 529 1947 9298 Mean (pg/ml) 456 1624 9289 529 1947 9298 Standard deviation 34.34 55.87 599.02 33.49 38.94 304.04 Standard deviation 34.34 55.87 599.02 33.49 38.94 304.04 CV (%) 7.5% 3.4% 6.4% 6.3% 2% 3.3% CV (%) 7.5% 3.4% 6.4% 6.3% 2% 3.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.11 0.155 0.254 0.479 0.841 1.418 2.047 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.11 0.155 0.254 0.479 0.841 1.418 2.047 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.036 0.11 0.155 0.254 0.479 0.841 1.418 2.047 O.D. 0.036 0.11 0.155 0.254 0.479 0.841 1.418 2.047

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.