Human FGF2 ELISA Kit PicoKine®

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 132 141 131 138 7.69 5.5% Sample 2 752 641 642 667 675 45.37 6.7% Sample 3 1076 1179 1055 1199 1127 62.59 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 132 141 131 138 7.69 5.5% Sample 1 150 132 141 131 138 7.69 5.5% Sample 2 752 641 642 667 675 45.37 6.7% Sample 2 752 641 642 667 675 45.37 6.7% Sample 3 1076 1179 1055 1199 1127 62.59 5.5% Sample 3 1076 1179 1055 1199 1127 62.59 5.5%

Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 752 1076 135 781 1071 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 CV (%) 7.3% 7.3% 7.4% 8% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 752 1076 135 781 1071 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 CV (%) 7.3% 7.3% 7.4% 8% 8% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 752 1076 135 781 1071 Mean (pg/ml) 150 752 1076 135 781 1071 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 CV (%) 7.3% 7.3% 7.4% 8% 8% 8.6% CV (%) 7.3% 7.3% 7.4% 8% 8% 8.6%

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER) /Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.057 0.117 0.169 0.26 0.51 0.87 1.427 1.838 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.057 0.117 0.169 0.26 0.51 0.87 1.427 1.838 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.057 0.117 0.169 0.26 0.51 0.87 1.427 1.838 O.D. 0.057 0.117 0.169 0.26 0.51 0.87 1.427 1.838

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.