Human alpha-defensin 1/DEFA1 ELISA Kit PicoKine®

Defensin is a human?protein?that is encoded by the?DEFA1?gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 429 405 424 430 20.53 4.7% Sample 2 1682 1777 1969 1748 1794 106.74 5.9% Sample 3 9496 8586 8746 8590 8854 375.94 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 429 405 424 430 20.53 4.7% Sample 1 462 429 405 424 430 20.53 4.7% Sample 2 1682 1777 1969 1748 1794 106.74 5.9% Sample 2 1682 1777 1969 1748 1794 106.74 5.9% Sample 3 9496 8586 8746 8590 8854 375.94 4.2% Sample 3 9496 8586 8746 8590 8854 375.94 4.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 1682 9496 635 1897 9984 Standard deviation 21.71 89.15 531.78 42.55 96.75 479.23 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 1682 9496 635 1897 9984 Standard deviation 21.71 89.15 531.78 42.55 96.75 479.23 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 462 1682 9496 635 1897 9984 Mean (pg/ml) 462 1682 9496 635 1897 9984 Standard deviation 21.71 89.15 531.78 42.55 96.75 479.23 Standard deviation 21.71 89.15 531.78 42.55 96.75 479.23 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8%

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.094 0.157 0.315 0.487 1.032 1.901 2.369 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.094 0.157 0.315 0.487 1.032 1.901 2.369 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.094 0.157 0.315 0.487 1.032 1.901 2.369 O.D. 0.031 0.094 0.157 0.315 0.487 1.032 1.901 2.369

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.