Human Dermatopontin/DPT ELISA Kit PicoKine®

Dermatopontin?is a?protein?that in humans is encoded by the?DPT?gene. It’s an?extracellular matrix?protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of?TGF-beta?through interaction with?decorin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 105 105 109 104 3.19 3% Sample 2 977 1016 907 886 946 52.39 5.5% Sample 3 2868 2711 2472 2675 2681 141.05 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 105 105 109 104 3.19 3% Sample 1 100 105 105 109 104 3.19 3% Sample 2 977 1016 907 886 946 52.39 5.5% Sample 2 977 1016 907 886 946 52.39 5.5% Sample 3 2868 2711 2472 2675 2681 141.05 5.2% Sample 3 2868 2711 2472 2675 2681 141.05 5.2%

Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 977 2868 91 933 3012 Standard deviation 7.1 46.89 169.21 7.91 58.77 234.93 CV (%) 7.1% 4.8% 5.9% 8.7% 6.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 977 2868 91 933 3012 Standard deviation 7.1 46.89 169.21 7.91 58.77 234.93 CV (%) 7.1% 4.8% 5.9% 8.7% 6.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 977 2868 91 933 3012 Mean (pg/ml) 100 977 2868 91 933 3012 Standard deviation 7.1 46.89 169.21 7.91 58.77 234.93 Standard deviation 7.1 46.89 169.21 7.91 58.77 234.93 CV (%) 7.1% 4.8% 5.9% 8.7% 6.3% 7.8% CV (%) 7.1% 4.8% 5.9% 8.7% 6.3% 7.8%

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.111 0.144 0.199 0.325 0.5973 1.249 1.935 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.111 0.144 0.199 0.325 0.5973 1.249 1.935 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.111 0.144 0.199 0.325 0.5973 1.249 1.935 O.D. 0.063 0.111 0.144 0.199 0.325 0.5973 1.249 1.935

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.