Mouse SCGB1A1/uteroglobin ELISA Kit PicoKine®

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 13 16 14 14 1.08 7.7% Sample 2 51 57 51 56 53 2.77 5.2% Sample 3 122 120 106 115 115 6.17 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 14 13 16 14 14 1.08 7.7% Sample 1 14 13 16 14 14 1.08 7.7% Sample 2 51 57 51 56 53 2.77 5.2% Sample 2 51 57 51 56 53 2.77 5.2% Sample 3 122 120 106 115 115 6.17 5.3% Sample 3 122 120 106 115 115 6.17 5.3%

Uteroglobin; Clara cell 17 kDa protein; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 51 122 13 47 124 Standard deviation 0.64 2.49 7.32 0.79 2.35 8.3 CV (%) 4.6% 4.9% 6% 6.1% 5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 14 51 122 13 47 124 Standard deviation 0.64 2.49 7.32 0.79 2.35 8.3 CV (%) 4.6% 4.9% 6% 6.1% 5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 14 51 122 13 47 124 Mean (pg/ml) 14 51 122 13 47 124 Standard deviation 0.64 2.49 7.32 0.79 2.35 8.3 Standard deviation 0.64 2.49 7.32 0.79 2.35 8.3 CV (%) 4.6% 4.9% 6% 6.1% 5% 6.7% CV (%) 4.6% 4.9% 6% 6.1% 5% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.009 0.059 0.138 0.312 0.6 1.104 1.664 1.897 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.009 0.059 0.138 0.312 0.6 1.104 1.664 1.897 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 Concentration (pg/ml) 0 4.7 9.4 18.8 37.5 75 150 300 O.D. 0.009 0.059 0.138 0.312 0.6 1.104 1.664 1.897 O.D. 0.009 0.059 0.138 0.312 0.6 1.104 1.664 1.897

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.