Rat HE4/WFDC2 ELISA Kit PicoKine®

Rat HE4/WFDC2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Wfdc2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

WAP four-disulfide core domain protein 2, also known as Human Epididymis Protein 4 (HE4), is a protein that in humans is encoded by the WFDC2 gene. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 295 325 303 331 313 14.92 4.7% Sample 2 1986 1810 2046 2132 1993 117.96 5.9% Sample 3 4547 4620 4129 4297 4398 196.24 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 295 325 303 331 313 14.92 4.7% Sample 1 295 325 303 331 313 14.92 4.7% Sample 2 1986 1810 2046 2132 1993 117.96 5.9% Sample 2 1986 1810 2046 2132 1993 117.96 5.9% Sample 3 4547 4620 4129 4297 4398 196.24 4.4% Sample 3 4547 4620 4129 4297 4398 196.24 4.4%

Synonyms

WAP four-disulfide core domain protein 2; Epididymal secretory protein 4; RE4; Wfdc2

Gene Name

Wfdc2

Gene ID

286888

UniProt

Q8CHN3

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E31-F168

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Markers, Tags & Cell Markers, Tumor Associated

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2316 1986 4547 316 2068 4341 Standard deviation 16.22 129.09 227.35 19.9 169.57 273.48 CV (%) 5.5% 6.5% 5% 8.2%% 8.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2316 1986 4547 316 2068 4341 Standard deviation 16.22 129.09 227.35 19.9 169.57 273.48 CV (%) 5.5% 6.5% 5% 8.2%% 8.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2316 1986 4547 316 2068 4341 Mean (pg/ml) 2316 1986 4547 316 2068 4341 Standard deviation 16.22 129.09 227.35 19.9 169.57 273.48 Standard deviation 16.22 129.09 227.35 19.9 169.57 273.48 CV (%) 5.5% 6.5% 5% 8.2%% 8.2% 6.3% CV (%) 5.5% 6.5% 5% 8.2%% 8.2% 6.3%

Sensitivity

<10 pg/mL

Function

Broad range protease inhibitor.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat HE4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

WAP four-disulfide core domain 2

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.121 0.158 0.226 0.369 0.629 1.199 2.178 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.121 0.158 0.226 0.369 0.629 1.199 2.178 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.121 0.158 0.226 0.369 0.629 1.199 2.178 O.D. 0.072 0.121 0.158 0.226 0.369 0.629 1.199 2.178

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.