Rat FABP3 ELISA Kit PicoKine®

Rat FABP3 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Fabp3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 127 121 131 127 4.09 3.2% Sample 2 866 923 850 898 884 28.26 3.1% Sample 3 2555 2241 2468 2352 2404 118.5 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 131 127 121 131 127 4.09 3.2% Sample 1 131 127 121 131 127 4.09 3.2% Sample 2 866 923 850 898 884 28.26 3.1% Sample 2 866 923 850 898 884 28.26 3.1% Sample 3 2555 2241 2468 2352 2404 118.5 4.9% Sample 3 2555 2241 2468 2352 2404 118.5 4.9%

Synonyms

Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3

Gene Name

Fabp3

Gene ID

79131

UniProt

P07483

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-A133

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Heart, but also skeletal muscle, kidney, brain and mammary gland.

Applications

ELISA

Field of Research

Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.7 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131 866 2555 124 868 2555 Mean (pg/ml) 131 866 2555 124 868 2555 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 Standard deviation 6.02 54.55 158.41 7.19 60.76 181.4 CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1% CV (%) 4.6% 7%% 6.2% 5.8% 7% 7.1%

Sensitivity

<10 pg/mL

Function

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat FABP3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fatty acid binding protein 3

Subcellular Location

Cytoplasm.

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06 O.D. 0.038 0.141 0.225 0.388 0.705 1.056 1.745 2.06

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.