Human AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine®

AGT (ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Sample 3 7370 7573 8346 7810 7774 364.72 4.6%

Angiotensinogen; Serpin A8; Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang I; Angiotensin-2; Angiotensin 1-8; Angiotensin II; Ang II; Angiotensin-3; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II; Angiotensin-4; Angiotensin 3-8; Angiotensin IV; Ang IV; Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4; AGT; SERPINA8

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.