Human AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine®

Human AGT/Serpin A8/Angiotensinogen ELISA Kit PicoKine® (96 Tests) . Quantitate Human AGT in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

AGT (ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 349 378 353 361 11.24 3.1% Sample 1 364 349 378 353 361 11.24 3.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 2 2379 2185 2390 2124 2269 117.06 5.1% Sample 3 7370 7573 8346 7810 7774 364.72 4.6% Sample 3 7370 7573 8346 7810 7774 364.72 4.6%

Synonyms

Angiotensinogen; Serpin A8; Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang I; Angiotensin-2; Angiotensin 1-8; Angiotensin II; Ang II; Angiotensin-3; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II; Angiotensin-4; Angiotensin 3-8; Angiotensin IV; Ang IV; Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4; AGT; SERPINA8

Gene Name

AGT

Gene ID

183

UniProt

P01019

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D34-A485

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Field of Research

Cardiovascular, G Protein Signaling, Signal Transduction, Signaling Pathway

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 2379 7370 417 2441 8736 Mean (pg/ml) 364 2379 7370 417 2441 8736 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 Standard deviation 18.93 126.09 405.35 25.44 156.22 585.31 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%

Sensitivity

<10 pg/mL

Function

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human AGT

Anticoagulant

EDTA

Gene Name Synonym

Angiotensinogen

Subcellular Location

Secreted.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114 O.D. 0.058 0.127 0.186 0.312 0.495 0.881 1.526 2.114

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.