Human NT-proANP/NPPA ELISA Kit PicoKine®

Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue) . ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney – that is, aldosterone stimulates sodium retention and it generates sodium loss.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 94 86 82 88 4.47 5% Sample 2 200 217 235 206 214 13.31 6.2% Sample 3 925 929 1045 879 944 61.25 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 94 86 82 88 4.47 5% Sample 1 90 94 86 82 88 4.47 5% Sample 2 200 217 235 206 214 13.31 6.2% Sample 2 200 217 235 206 214 13.31 6.2% Sample 3 925 929 1045 879 944 61.25 6.4% Sample 3 925 929 1045 879 944 61.25 6.4%

Natriuretic peptides A; CDD-ANF; Cardiodilatin; CDD; Cardiodilatin-related peptide; CDP; Prepronatriodilatin; Atrial natriuretic factor; ANF; Atrial natriuretic peptide; ANP; NPPA; ANP; PND

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 200 925 91 182 898 Standard deviation 4.23 13.8 52.72 5.46 15.83 66.45 CV (%) 4.7% 6.9% 5.7% 6% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 200 925 91 182 898 Standard deviation 4.23 13.8 52.72 5.46 15.83 66.45 CV (%) 4.7% 6.9% 5.7% 6% 8.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 200 925 91 182 898 Mean (pg/ml) 90 200 925 91 182 898 Standard deviation 4.23 13.8 52.72 5.46 15.83 66.45 Standard deviation 4.23 13.8 52.72 5.46 15.83 66.45 CV (%) 4.7% 6.9% 5.7% 6% 8.7% 7.4% CV (%) 4.7% 6.9% 5.7% 6% 8.7% 7.4%

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.082 0.169 0.225 0.311 0.496 0.819 1.297 2.064 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.082 0.169 0.225 0.311 0.496 0.819 1.297 2.064 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.082 0.169 0.225 0.311 0.496 0.819 1.297 2.064 O.D. 0.082 0.169 0.225 0.311 0.496 0.819 1.297 2.064

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.