Human IL-19/Interleukin-19 ELISA Kit PicoKine®

Human IL-19 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL19 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 19 (IL19) is a protein that in humans is encoded by the IL19 gene. The protein encoded by this gene is a cytokine that belongs to the IL-10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the interleukin-20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3) . A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 58 51 55 55 2.68 4.8% Sample 2 290 309 293 295 296 7.29 2.4% Sample 3 1018 1068 897 1026 1002 63.66 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 58 51 55 55 2.68 4.8% Sample 1 57 58 51 55 55 2.68 4.8% Sample 2 290 309 293 295 296 7.29 2.4% Sample 2 290 309 293 295 296 7.29 2.4% Sample 3 1018 1068 897 1026 1002 63.66 6.3% Sample 3 1018 1068 897 1026 1002 63.66 6.3%

Synonyms

Interleukin-19; IL-19; Melanoma differentiation-associated protein-like protein; NG.1; IL19; ZMDA1

Gene Name

IL19

Gene ID

29949

UniProt

Q9UHD0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-A177

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 290 1018 55 290 920 Standard deviation 2.5 21.17 61.08 3.35 22.04 66.24 CV (%) 4.4% 7.3% 6% 6.1% 7.6% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 290 1018 55 290 920 Standard deviation 2.5 21.17 61.08 3.35 22.04 66.24 CV (%) 4.4% 7.3% 6% 6.1% 7.6% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 290 1018 55 290 920 Mean (pg/ml) 57 290 1018 55 290 920 Standard deviation 2.5 21.17 61.08 3.35 22.04 66.24 Standard deviation 2.5 21.17 61.08 3.35 22.04 66.24 CV (%) 4.4% 7.3% 6% 6.1% 7.6% 7.2% CV (%) 4.4% 7.3% 6% 6.1% 7.6% 7.2%

Sensitivity

<10 pg/mL

Function

May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human IL-19

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin 19

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.082 0.124 0.197 0.319 0.579 1.049 1.947 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.082 0.124 0.197 0.319 0.579 1.049 1.947 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.082 0.124 0.197 0.319 0.579 1.049 1.947 O.D. 0.036 0.082 0.124 0.197 0.319 0.579 1.049 1.947

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.