Human Vitamin D BP/GC/Vitamin D Binding Protein ELISA Kit PicoKine®

Human Vitamin DBP ELISA Kit PicoKine® (96 Tests) . Quantitate Human GC in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vitamin D-binding protein, also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene.The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2%

Synonyms

Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor

Gene Name

Gene ID

2638

UniProt

P02774

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L17-L474

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.

Applications

ELISA

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 72578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 72578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 72578.68 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 72578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2%

Sensitivity

<150 pg/mL

Function

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Vitamin DBP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

GC, vitamin D binding protein

Subcellular Location

Secreted.

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.034 0.211 0.283 0.373 0.611 1.025 1.622 2.382 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.034 0.211 0.283 0.373 0.611 1.025 1.622 2.382 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.034 0.211 0.283 0.373 0.611 1.025 1.622 2.382 O.D. 0.034 0.211 0.283 0.373 0.611 1.025 1.622 2.382

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.