Human MCAM/CD146 ELISA Kit PicoKine®

CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. By radiation hybrid analysis, it mapped the CD146 gene to chromosome 11q23.3.It is a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 158 162 156 154 7.11 4.6% Sample 2 548 529 527 526 532 9.01 1.6% Sample 3 1021 1095 1036 1044 1049 27.81 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 158 162 156 154 7.11 4.6% Sample 1 143 158 162 156 154 7.11 4.6% Sample 2 548 529 527 526 532 9.01 1.6% Sample 2 548 529 527 526 532 9.01 1.6% Sample 3 1021 1095 1036 1044 1049 27.81 2.6% Sample 3 1021 1095 1036 1044 1049 27.81 2.6%

Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146; MCAM; MUC18

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Cancer, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Invasion/Microenvironment, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 548 1021 131 561 1060 Standard deviation 6 23.56 62.28 7.07 31.97 80.56 CV (%) 4.2% 4.3% 6.1% 5.4% 5.7% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 548 1021 131 561 1060 Standard deviation 6 23.56 62.28 7.07 31.97 80.56 CV (%) 4.2% 4.3% 6.1% 5.4% 5.7% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 548 1021 131 561 1060 Mean (pg/ml) 143 548 1021 131 561 1060 Standard deviation 6 23.56 62.28 7.07 31.97 80.56 Standard deviation 6 23.56 62.28 7.07 31.97 80.56 CV (%) 4.2% 4.3% 6.1% 5.4% 5.7% 7.6% CV (%) 4.2% 4.3% 6.1% 5.4% 5.7% 7.6%

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.153 0.245 0.396 0.735 1.139 1.715 2.121 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.153 0.245 0.396 0.735 1.139 1.715 2.121 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.153 0.245 0.396 0.735 1.139 1.715 2.121 O.D. 0.051 0.153 0.245 0.396 0.735 1.139 1.715 2.121

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.