Human CDCP1 ELISA Kit PicoKine®

CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases) . CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 53 62 62 57 4.26 7.4% Sample 2 307 305 306 280 299 11.28 3.7% Sample 3 865 848 944 941 899 43.43 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 53 62 62 57 4.26 7.4% Sample 1 54 53 62 62 57 4.26 7.4% Sample 2 307 305 306 280 299 11.28 3.7% Sample 2 307 305 306 280 299 11.28 3.7% Sample 3 865 848 944 941 899 43.43 4.8% Sample 3 865 848 944 941 899 43.43 4.8%

CUB domain-containing protein 1; Membrane glycoprotein gp140; Subtractive immunization M plus HEp3-associated 135 kDa protein; SIMA135; Transmembrane and associated with src kinases; CD318; CDCP1; TRASK; UNQ2486/PRO5773

Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

Cancer, Cell Type Markers, Extracellular Matrix, Hematopoietic Progenitors, Invasion/Microenvironment, Stem Cells, Surface Molecules, Tags & Cell Markers, Tumor Associated, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 307 865 54 291 945 Standard deviation 4.26 12.89 62.28 4.59 14.84 68.98 CV (%) 7.9% 4.2% 7.2% 8.5% 7.3%% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 307 865 54 291 945 Standard deviation 4.26 12.89 62.28 4.59 14.84 68.98 CV (%) 7.9% 4.2% 7.2% 8.5% 7.3%% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 307 865 54 291 945 Mean (pg/ml) 54 307 865 54 291 945 Standard deviation 4.26 12.89 62.28 4.59 14.84 68.98 Standard deviation 4.26 12.89 62.28 4.59 14.84 68.98 CV (%) 7.9% 4.2% 7.2% 8.5% 7.3%% 7.3% CV (%) 7.9% 4.2% 7.2% 8.5% 7.3%% 7.3%

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.077 0.123 0.21 0.402 0.751 1.432 2.444 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.077 0.123 0.21 0.402 0.751 1.432 2.444 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.077 0.123 0.21 0.402 0.751 1.432 2.444 O.D. 0.025 0.077 0.123 0.21 0.402 0.751 1.432 2.444

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.