Human Serpin G1 ELISA Kit PicoKine®

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE) . Alternative splicing results in multiple transcript variants encoding the same isoform.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 209 218 225 15.73 6.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 3 6095 6211 6061 5414 5945 311.71 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 222 251 209 218 225 15.73 6.9% Sample 1 222 251 209 218 225 15.73 6.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 2 1297 1249 1215 1306 1266 36.9 2.9% Sample 3 6095 6211 6061 5414 5945 311.71 5.2% Sample 3 6095 6211 6061 5414 5945 311.71 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 222 1297 60242 242 1418 6290 Mean (pg/ml) 222 1297 60242 242 1418 6290 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 Standard deviation 13.98 63.55 365.7 18.39 95 377.4 CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6% CV (%) 6.7% 4.9% 6% 7.6% 6.7% 6%

Activation of the C1 complex is under control of the C1- inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201 O.D. 0.042 0.116 0.18 0.309 0.521 0.861 1.448 2.201

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.