Human IL-38/IL1F10 ELISA Kit PicoKine®

Interleukin-1 family member 10 is a protein that in humans is encoded by the IL1F10 gene. The protein encoded by thi s gene is a member of the interleukin 1 cytokine family. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 215 206 177 200 14.13 7% Sample 2 449 456 455 464 456 5.33 1.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 215 206 177 200 14.13 7% Sample 1 203 215 206 177 200 14.13 7% Sample 2 449 456 455 464 456 5.33 1.1% Sample 2 449 456 455 464 456 5.33 1.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1% Sample 3 1984 2038 1885 1780 1921 98.53 5.1%

Interleukin-1 family member 10; IL-1F10; Family of interleukin 1-theta; FIL1 theta; Interleukin-1 HY2; IL-1HY2; Interleukin-1 theta; IL-1 theta; Interleukin-38; IL-38; IL1F10; FIL1T; IL1HY2; IL38; FKSG75; UNQ6119/PRO20041

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 449 1984 197 483 1980 Mean (pg/ml) 203 449 1984 197 483 1980 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 Standard deviation 12.58 26.94 87.29 13.98 38.15 99 CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5% CV (%) 6.2% 6% 4.4% 7.1% 7.9% 5%

Cytokine with immunomodulatory activity. Alone, does not induce cytokine production, but reduces IL22 and IL17A production by T-cells in response to heat-killed Candida albicans. Reduces IL36G-induced production of IL8 by peripheral blood mononuclear cells. Increases IL6 production by dendritic cells stimulated by bacterial lipopolysaccharides (LPS) . Ligand for IL-36R/IL1RL2.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428 O.D. 0.061 0.119 0.175 0.296 0.52 0.952 1.608 2.428

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.