Human IL15RA ELISA Kit PicoKine®

Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 258 255 282 272 16.34 6% Sample 2 1273 1294 1280 1119 1241 71.12 5.7% Sample 3 4114 4965 4963 4181 4555 408.93 8.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 294 258 255 282 272 16.34 6% Sample 1 294 258 255 282 272 16.34 6% Sample 2 1273 1294 1280 1119 1241 71.12 5.7% Sample 2 1273 1294 1280 1119 1241 71.12 5.7% Sample 3 4114 4965 4963 4181 4555 408.93 8.9% Sample 3 4114 4965 4963 4181 4555 408.93 8.9%

Interleukin-15 receptor subunit alpha; IL-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; CD215; Soluble interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; sIL-15R-alpha; sIL-15RA; IL15RA

Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 1273 4114 317 1160 3857 Standard deviation 20.87 824.43 193.35 24.4 97.44 239.13 CV (%) 7.1% 6.9% 4.7% 7.7% 8.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 294 1273 4114 317 1160 3857 Standard deviation 20.87 824.43 193.35 24.4 97.44 239.13 CV (%) 7.1% 6.9% 4.7% 7.7% 8.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 294 1273 4114 317 1160 3857 Mean (pg/ml) 294 1273 4114 317 1160 3857 Standard deviation 20.87 824.43 193.35 24.4 97.44 239.13 Standard deviation 20.87 824.43 193.35 24.4 97.44 239.13 CV (%) 7.1% 6.9% 4.7% 7.7% 8.4% 6.2% CV (%) 7.1% 6.9% 4.7% 7.7% 8.4% 6.2%

High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves SYK.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.218 0.337 0.549 0.869 1.376 1.921 2.229 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.218 0.337 0.549 0.869 1.376 1.921 2.229 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.218 0.337 0.549 0.869 1.376 1.921 2.229 O.D. 0.07 0.218 0.337 0.549 0.869 1.376 1.921 2.229

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.