Human SMOC2 ELISA Kit PicoKine®

SPARC-related modular calcium-binding protein 2 is a protein that in humans is encoded by the SMOC2 gene. This gene encodes a member of the SPARC family (secreted protein acidic and rich in cysteine/osteonectin/BM-40), which are highly expressed during embryogenesis and wound healing. The gene product is a matricellular protein which promotes matrix assembly and can stimulate endothelial cell proliferation and migration, as well as angiogenic activity. Associated with pulmonary function, this secretory gene product contains a Kazal domain, two thymoglobulin type-1 domains, and two EF-hand calcium-binding domains. The encoded protein may serve as a target for controlling angiogenesis in tumor growth and myocardial ischemia. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 143 136 132 138 4.49 3.2% Sample 2 785 719 731 758 748 25.48 3.4% Sample 3 1622 1861 1845 1879 1801 104.47 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 143 136 132 138 4.49 3.2% Sample 1 142 143 136 132 138 4.49 3.2% Sample 2 785 719 731 758 748 25.48 3.4% Sample 2 785 719 731 758 748 25.48 3.4% Sample 3 1622 1861 1845 1879 1801 104.47 5.8% Sample 3 1622 1861 1845 1879 1801 104.47 5.8%

SPARC-related modular calcium-binding protein 2; Secreted modular calcium-binding protein 2; SMOC-2; Smooth muscle-associated protein 2; SMAP-2; SMOC2; SMAP2; MSTP117

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 785 1622 131 825 1562 Standard deviation 8.09 51.02 128.13 8.38 56.92 148.39 CV (%) 5.7% 6.5% 7.9% 6.4% 6.9% 9.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 785 1622 131 825 1562 Standard deviation 8.09 51.02 128.13 8.38 56.92 148.39 CV (%) 5.7% 6.5% 7.9% 6.4% 6.9% 9.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 785 1622 131 825 1562 Mean (pg/ml) 142 785 1622 131 825 1562 Standard deviation 8.09 51.02 128.13 8.38 56.92 148.39 Standard deviation 8.09 51.02 128.13 8.38 56.92 148.39 CV (%) 5.7% 6.5% 7.9% 6.4% 6.9% 9.5% CV (%) 5.7% 6.5% 7.9% 6.4% 6.9% 9.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.12 0.203 0.349 0.618 1.077 1.812 2.431 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.12 0.203 0.349 0.618 1.077 1.812 2.431 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.12 0.203 0.349 0.618 1.077 1.812 2.431 O.D. 0.05 0.12 0.203 0.349 0.618 1.077 1.812 2.431

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.