Mouse TRKC ELISA Kit PicoKine®

NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 311 356 360 342 19.25 5.6% Sample 2 1397 1206 1266 1192 1265 80.98 6.4% Sample 3 4298 4403 4329 3717 4186 273.87 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 344 311 356 360 342 19.25 5.6% Sample 1 344 311 356 360 342 19.25 5.6% Sample 2 1397 1206 1266 1192 1265 80.98 6.4% Sample 2 1397 1206 1266 1192 1265 80.98 6.4% Sample 3 4298 4403 4329 3717 4186 273.87 6.5% Sample 3 4298 4403 4329 3717 4186 273.87 6.5%

Isoform 2 expression is restricted to specific areas in adult brain. Isoform 3 transcripts are readily detected early during embryogenesis and are expressed predominantly in adult brain and gonads.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1397 4298 373 1457 4638 Standard deviation 22.01 92.2 197.7 27.22 104.9 241.17 CV (%) 6.4% 6.6% 4.6% 7.3% 7.2% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 344 1397 4298 373 1457 4638 Standard deviation 22.01 92.2 197.7 27.22 104.9 241.17 CV (%) 6.4% 6.6% 4.6% 7.3% 7.2% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 344 1397 4298 373 1457 4638 Mean (pg/ml) 344 1397 4298 373 1457 4638 Standard deviation 22.01 92.2 197.7 27.22 104.9 241.17 Standard deviation 22.01 92.2 197.7 27.22 104.9 241.17 CV (%) 6.4% 6.6% 4.6% 7.3% 7.2% 5.2% CV (%) 6.4% 6.6% 4.6% 7.3% 7.2% 5.2%

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.087 0.129 0.198 0.478 0.991 1.817 2.424 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.087 0.129 0.198 0.478 0.991 1.817 2.424 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.066 0.087 0.129 0.198 0.478 0.991 1.817 2.424 O.D. 0.066 0.087 0.129 0.198 0.478 0.991 1.817 2.424

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.