Human DAND5/COCO ELISA Kit PicoKine®

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 165 155 172 164 6.04 3.6% Sample 2 636 669 669 568 635 41.23 6.4% Sample 3 1889 1827 1878 1962 1889 48.2 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 165 155 172 164 6.04 3.6% Sample 1 164 165 155 172 164 6.04 3.6% Sample 2 636 669 669 568 635 41.23 6.4% Sample 2 636 669 669 568 635 41.23 6.4% Sample 3 1889 1827 1878 1962 1889 48.2 2.5% Sample 3 1889 1827 1878 1962 1889 48.2 2.5%

DAN domain family member 5; Cerberus-like protein 2; Cerl-2; Cysteine knot superfamily 1, BMP antagonist 3; Gremlin-3; DAND5; CER2; CKTSF1B3; GREM3; SP1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 636 1889 151 643 1700 Standard deviation 10.66 43.88 102 10.57 50.79 96.9 CV (%) 6.5% 6.9% 5.4% 7% 7.9% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 636 1889 151 643 1700 Standard deviation 10.66 43.88 102 10.57 50.79 96.9 CV (%) 6.5% 6.9% 5.4% 7% 7.9% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 164 636 1889 151 643 1700 Mean (pg/ml) 164 636 1889 151 643 1700 Standard deviation 10.66 43.88 102 10.57 50.79 96.9 Standard deviation 10.66 43.88 102 10.57 50.79 96.9 CV (%) 6.5% 6.9% 5.4% 7% 7.9% 5.7% CV (%) 6.5% 6.9% 5.4% 7% 7.9% 5.7%

Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.107 0.163 0.27 0.438 0.771 1.336 1.859 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.107 0.163 0.27 0.438 0.771 1.336 1.859 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.035 0.107 0.163 0.27 0.438 0.771 1.336 1.859 O.D. 0.035 0.107 0.163 0.27 0.438 0.771 1.336 1.859

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.