Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine®

Human DKK-4 / Dickkopf 4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DKK4 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Reporter gene

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 234 223 250 236 9.65 4% Sample 2 1092 965 1125 1149 1082 70.93 6.5% Sample 3 4513 4306 4063 4001 4220 203.62 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 234 223 250 236 9.65 4% Sample 1 238 234 223 250 236 9.65 4% Sample 2 1092 965 1125 1149 1082 70.93 6.5% Sample 2 1092 965 1125 1149 1082 70.93 6.5% Sample 3 4513 4306 4063 4001 4220 203.62 4.8% Sample 3 4513 4306 4063 4001 4220 203.62 4.8%

Synonyms

Dickkopf-related protein 4; Dickkopf-4; Dkk-4; hDkk-4; Dickkopf-related protein 4 short form; DKK4

Gene Name

DKK4

Gene ID

27121

UniProt

Q9UBT3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-L224

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in cerebellum, T-cells, esophagus and lung.

Applications

ELISA

Field of Research

Secreted, Signaling Pathways, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1092 4513 249 988 4453 Standard deviation 14.99 519.427 342.98 19.42 55.32 395.6%1 CV (%) 5.6%% 5.3% 7.6% 7.8% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1092 4513 249 988 4453 Standard deviation 14.99 519.427 342.98 19.42 55.32 395.6%1 CV (%) 5.6%% 5.3% 7.6% 7.8% 5.6% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 238 1092 4513 249 988 4453 Mean (pg/ml) 238 1092 4513 249 988 4453 Standard deviation 14.99 519.427 342.98 19.42 55.32 395.6%1 Standard deviation 14.99 519.427 342.98 19.42 55.32 395.6%1 CV (%) 5.6%% 5.3% 7.6% 7.8% 5.6% 8.9% CV (%) 5.6%% 5.3% 7.6% 7.8% 5.6% 8.9%

Sensitivity

<10 pg/mL

Function

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human DKK-4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Dickkopf WNT signaling pathway inhibitor 4

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.071 0.105 0.177 0.313 0.569 1.059 1.881 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.071 0.105 0.177 0.313 0.569 1.059 1.881 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.071 0.105 0.177 0.313 0.569 1.059 1.881 O.D. 0.047 0.071 0.105 0.177 0.313 0.569 1.059 1.881

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.