Rat CXCL4/PF4 ELISA Kit PicoKine®

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4) . The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 211 226 204 211 8.78 4.1% Sample 2 1658 1447 1691 1522 1579 99.31 6.2% Sample 3 5065 4548 5213 4497 4830 313.17 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 205 211 226 204 211 8.78 4.1% Sample 1 205 211 226 204 211 8.78 4.1% Sample 2 1658 1447 1691 1522 1579 99.31 6.2% Sample 2 1658 1447 1691 1522 1579 99.31 6.2% Sample 3 5065 4548 5213 4497 4830 313.17 6.4% Sample 3 5065 4548 5213 4497 4830 313.17 6.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1658 5065 199 1676 4964 Standard deviation 8.81 72.95 379.87 9.35 87.15 416.97 CV (%) 4.3% 4.4% 7.5% 4.7% 5.2% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 205 1658 5065 199 1676 4964 Standard deviation 8.81 72.95 379.87 9.35 87.15 416.97 CV (%) 4.3% 4.4% 7.5% 4.7% 5.2% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 205 1658 5065 199 1676 4964 Mean (pg/ml) 205 1658 5065 199 1676 4964 Standard deviation 8.81 72.95 379.87 9.35 87.15 416.97 Standard deviation 8.81 72.95 379.87 9.35 87.15 416.97 CV (%) 4.3% 4.4% 7.5% 4.7% 5.2% 8.4% CV (%) 4.3% 4.4% 7.5% 4.7% 5.2% 8.4%

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.049 0.072 0.12 0.226 0.442 0.923 1.874 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.049 0.072 0.12 0.226 0.442 0.923 1.874 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.049 0.072 0.12 0.226 0.442 0.923 1.874 O.D. 0.01 0.049 0.072 0.12 0.226 0.442 0.923 1.874

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.