Human Hyaluronidase1/HYAL1 ELISA Kit PicoKine®

Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 72 75 79 73 0.95 1.3% Sample 2 223 242 236 225 232 9.95 4.3% Sample 3 399 421 416 422 415 29.84 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 72 75 79 73 0.95 1.3% Sample 1 67 72 75 79 73 0.95 1.3% Sample 2 223 242 236 225 232 9.95 4.3% Sample 2 223 242 236 225 232 9.95 4.3% Sample 3 399 421 416 422 415 29.84 7.2% Sample 3 399 421 416 422 415 29.84 7.2%

Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 223 399 72 204 415 Standard deviation 4.42 14.49 21.14 5.26 14.07 26.14 CV (%) 6.6% 6.5% 5.3% 7.3% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 223 399 72 204 415 Standard deviation 4.42 14.49 21.14 5.26 14.07 26.14 CV (%) 6.6% 6.5% 5.3% 7.3% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 67 223 399 72 204 415 Mean (pg/ml) 67 223 399 72 204 415 Standard deviation 4.42 14.49 21.14 5.26 14.07 26.14 Standard deviation 4.42 14.49 21.14 5.26 14.07 26.14 CV (%) 6.6% 6.5% 5.3% 7.3% 6.9% 6.3% CV (%) 6.6% 6.5% 5.3% 7.3% 6.9% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.038 0.106 0.179 0.281 0.499 0.845 1.346 1.829 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.038 0.106 0.179 0.281 0.499 0.845 1.346 1.829 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.038 0.106 0.179 0.281 0.499 0.845 1.346 1.829 O.D. 0.038 0.106 0.179 0.281 0.499 0.845 1.346 1.829

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.