Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®

Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7%

Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2) ; PAF 2-acylhydrolase; PLA2G7; PAFAH

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7%

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.