Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine®

Human PLA2G7/Lp-PLA2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human PLA2G7 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45-kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 1 1179 1334 1212 1128 1213 75.86 6.2% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 2 7607 7415 7511 7063 7399 205.52 2.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7% Sample 3 22955 22970 21188 24226 22834 1081.67 4.7%

Synonyms

Platelet-activating factor acetylhydrolase; PAF acetylhydrolase; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA (2) ; PAF 2-acylhydrolase; PLA2G7; PAFAH

Gene Name

PLA2G7

Gene ID

7941

UniProt

Q13093

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F22-N441

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Tissue Specificity

Plasma.

Applications

ELISA

Field of Research

Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Platelets, Secreted Molecules

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Mean (pg/ml) 1179 7607 2212395 1239 6920 22984 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 Standard deviation 55.41 418.38 1124.79 75.57 456.72 1539.92 CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7% CV (%) 4.7% 5.5% 4.9% 6.1% 6.6% 6.7%

Sensitivity

<20 pg/mL

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PLA2G7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Phospholipase A2 group VII

Subcellular Location

Secreted, extracellular space.

Protein Name

Platelet-activating factor acetylhydrolase

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028 O.D. 0.063 0.135 0.217 0.403 0.679 1.05 1.612 2.028

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.