Human CD209 ELISA Kit PicoKine®

DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) also known as CD209 (Cluster of Differentiation 209) is a protein which in humans is encoded by the CD209 gene. This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene. DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 157 153 153 158 7.39 4.6% Sample 2 628 650 644 600 630 19.35 3% Sample 3 2962 3138 2784 3082 2991 135.62 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 157 153 153 158 7.39 4.6% Sample 1 171 157 153 153 158 7.39 4.6% Sample 2 628 650 644 600 630 19.35 3% Sample 2 628 650 644 600 630 19.35 3% Sample 3 2962 3138 2784 3082 2991 135.62 4.5% Sample 3 2962 3138 2784 3082 2991 135.62 4.5%

Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.

Adaptive Immunity, Antiviral Signaling, Cell Adhesion, Cytoskeleton/ECM, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology, Signal Transduction, T Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 628 2962 178 619 3253 Standard deviation 6.84 25.74 148.1 9.79 28.47 185.42 CV (%) 4% 4.1% 5% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 628 2962 178 619 3253 Standard deviation 6.84 25.74 148.1 9.79 28.47 185.42 CV (%) 4% 4.1% 5% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 171 628 2962 178 619 3253 Mean (pg/ml) 171 628 2962 178 619 3253 Standard deviation 6.84 25.74 148.1 9.79 28.47 185.42 Standard deviation 6.84 25.74 148.1 9.79 28.47 185.42 CV (%) 4% 4.1% 5% 5.5% 4.6% 5.7% CV (%) 4% 4.1% 5% 5.5% 4.6% 5.7%

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.0 0.092 0.191 0.371 0.647 1.084 1.595 2.17 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.0 0.092 0.191 0.371 0.647 1.084 1.595 2.17 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.0 0.092 0.191 0.371 0.647 1.084 1.595 2.17 O.D. 0.0 0.092 0.191 0.371 0.647 1.084 1.595 2.17

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.