Human Bikunin / alpha 1 Microglobulin ELISA Kit PicoKine®

Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 35 40 38 2.04 5.3% Sample 2 243 252 272 236 250 13.51 5.4% Sample 3 418 447 460 414 434 19.35 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 40 35 40 38 2.04 5.3% Sample 1 38 40 35 40 38 2.04 5.3% Sample 2 243 252 272 236 250 13.51 5.4% Sample 2 243 252 272 236 250 13.51 5.4% Sample 3 418 447 460 414 434 19.35 4.4% Sample 3 418 447 460 414 434 19.35 4.4%

Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; AMBP; HCP; ITIL

Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 38 243 418 36 264 406 Mean (pg/ml) 38 243 418 36 264 406 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 Standard deviation 2.35 16.03 25.08 2.48 18.48 26.2%7 CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2% CV (%) 6.2% 6.6% 6% 6.9% 7% 6.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868 O.D. 0.082 0.148 0.199 0.325 0.472 0.769 1.218 1.868

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.