Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What’s more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1435 1482 1554 1340 1452 77.67 5.3% Sample 2 4423 4063 4082 3952 4130 176.29 4.2% Sample 3 19418 20714 18137 20289 19639 985.26 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1435 1482 1554 1340 1452 77.67 5.3% Sample 1 1435 1482 1554 1340 1452 77.67 5.3% Sample 2 4423 4063 4082 3952 4130 176.29 4.2% Sample 2 4423 4063 4082 3952 4130 176.29 4.2% Sample 3 19418 20714 18137 20289 19639 985.26 5% Sample 3 19418 20714 18137 20289 19639 985.26 5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1435 4423 19418 1328 4783 18803 Standard deviation 107.62 309.61 1378.67 111.55 411.33 1541.84 CV (%) 7.5% 7% 7.1% 8.4% 8.6% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1435 4423 19418 1328 4783 18803 Standard deviation 107.62 309.61 1378.67 111.55 411.33 1541.84 CV (%) 7.5% 7% 7.1% 8.4% 8.6% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1435 4423 19418 1328 4783 18803 Mean (pg/ml) 1435 4423 19418 1328 4783 18803 Standard deviation 107.62 309.61 1378.67 111.55 411.33 1541.84 Standard deviation 107.62 309.61 1378.67 111.55 411.33 1541.84 CV (%) 7.5% 7% 7.1% 8.4% 8.6% 8.2% CV (%) 7.5% 7% 7.1% 8.4% 8.6% 8.2%

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.067 0.087 0.147 0.289 0.537 1.066 2.2 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.067 0.087 0.147 0.289 0.537 1.066 2.2 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.067 0.087 0.147 0.289 0.537 1.066 2.2 O.D. 0.037 0.067 0.087 0.147 0.289 0.537 1.066 2.2

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.