Mouse IL-24/Interleukin-24 ELISA Kit PicoKine®

Interleukin 24 (IL-24) is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as Melanoma differentiation-associated 7 (mda-7) due to its discovery as a tumour suppressing protein. By radiation hybrid analysis, the MDA7 gene is mapped to 1q32.2-q41. IL-24 appears to control in cell survival and proliferation by inducing rapid activation of particular transcription factors called Stat-1 and Stat-3. Mouse IL24 was expressed as an approximately 35-kD protein that was converted to a 23-kD protein by deglycosylation. The IL24 protein shares 24 to 33% homology with other members of the IL10 family of cytokines, with highest homology with IL20. This cytokine is predominantly released by activated monocytes, macrophages and T helper 2 (Th2) cells and acts on non-haematopoietic tissues such as skin, lung and reproductive tissues. IL-24 performs important roles in wound healing, psoriasis and cancer.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Sample 3 1654 1616 1741 1741 1688 54.67 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 Standard deviation 8.94 25.53 77.73 5.7%%5 29.98 90.86 CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5% CV (%) 5.7%% 4.6% 4.7% 5.7%% 5.7% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04 O.D. 0.084 0.129 0.171 0.233 0.391 0.712 1.32 2.04

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.