Human ICOS ELISA Kit PicoKine®

Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 189 191 192 190 1.29 0.6% Sample 2 561 545 489 526 530 26.84 5% Sample 3 1066 1100 974 1134 1068 59.62 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 189 191 192 190 1.29 0.6% Sample 1 189 189 191 192 190 1.29 0.6% Sample 2 561 545 489 526 530 26.84 5% Sample 2 561 545 489 526 530 26.84 5% Sample 3 1066 1100 974 1134 1068 59.62 5.5% Sample 3 1066 1100 974 1134 1068 59.62 5.5%

Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B- cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 189 561 1066 198 531 1149 Mean (pg/ml) 189 561 1066 198 531 1149 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 Standard deviation 13.41 22.44 52.23 16.83 27.61 58.59 CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1% CV (%) 7.1% 4% 4.9% 8.5% 5.2% 5.1%

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817 O.D. 0.043 0.081 0.137 0.215 0.406 0.714 1.226 1.817

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.