Mouse PLGF-2 / PGF ELISA Kit PicoKine®

Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 40 36 33 35 2.87 8.2% Sample 2 176 178 189 176 179 5.4 3% Sample 3 474 405 411 424 428 27.15 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 40 36 33 35 2.87 8.2% Sample 1 33 40 36 33 35 2.87 8.2% Sample 2 176 178 189 176 179 5.4 3% Sample 2 176 178 189 176 179 5.4 3% Sample 3 474 405 411 424 428 27.15 6.3% Sample 3 474 405 411 424 428 27.15 6.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 176 474 34 182 481 Standard deviation 2.01 12.14 20.38 2.24 13.83 20.68 CV (%) 6.1% 6.9% 4.3% 6.6% 7.6% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 176 474 34 182 481 Standard deviation 2.01 12.14 20.38 2.24 13.83 20.68 CV (%) 6.1% 6.9% 4.3% 6.6% 7.6% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 176 474 34 182 481 Mean (pg/ml) 33 176 474 34 182 481 Standard deviation 2.01 12.14 20.38 2.24 13.83 20.68 Standard deviation 2.01 12.14 20.38 2.24 13.83 20.68 CV (%) 6.1% 6.9% 4.3% 6.6% 7.6% 4.3% CV (%) 6.1% 6.9% 4.3% 6.6% 7.6% 4.3%

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.194 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.194 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.194 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.194

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.