Rat CXCL7/PPBP ELISA Kit PicoKine®

Rat PPBP/CXCL7 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Ppbp in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3) . It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 36 36 35 1.5 4.2% Sample 2 190 203 205 198 199 5.78 2.9% Sample 3 491 492 496 465 486 12.26 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 37 36 36 35 1.5 4.2% Sample 1 33 37 36 36 35 1.5 4.2% Sample 2 190 203 205 198 199 5.78 2.9% Sample 2 190 203 205 198 199 5.78 2.9% Sample 3 491 492 496 465 486 12.26 2.5% Sample 3 491 492 496 465 486 12.26 2.5%

Synonyms

CXC chemokine RTCK1

Gene Name

Ppbp

Gene ID

246358

UniProt

Q99ME0

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: K38-Y111

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 190 491 32 177 470 Mean (pg/ml) 33 190 491 32 177 470 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 Standard deviation 2.11 10.64 25.04 2.65 12.56 29.61 CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3% CV (%) 6.4% 5.6% 6.3%% 8.3% 7.1% 6.3%

Sensitivity

<2 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat CXCL7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Pro-platelet basic protein

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9 O.D. 0.001 0.065 0.138 0.303 0.53 1.091 1.601 1.9

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.