Mouse CXCL7/PPBP ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3) . It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 35 32 35 2.04 5.8% Sample 2 173 164 153 159 162 7.32 4.5% Sample 3 408 466 453 448 443 21.66 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 37 35 32 35 2.04 5.8% Sample 1 37 37 35 32 35 2.04 5.8% Sample 2 173 164 153 159 162 7.32 4.5% Sample 2 173 164 153 159 162 7.32 4.5% Sample 3 408 466 453 448 443 21.66 4.8% Sample 3 408 466 453 448 443 21.66 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 173 408 33 166 406 Standard deviation 1.96 11.76 16.72 2.17 11.62 17.05 CV (%) 5.3% 6.8% 4.1% 6.6% 7% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 173 408 33 166 406 Standard deviation 1.96 11.76 16.72 2.17 11.62 17.05 CV (%) 5.3% 6.8% 4.1% 6.6% 7% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 173 408 33 166 406 Mean (pg/ml) 37 173 408 33 166 406 Standard deviation 1.96 11.76 16.72 2.17 11.62 17.05 Standard deviation 1.96 11.76 16.72 2.17 11.62 17.05 CV (%) 5.3% 6.8% 4.1% 6.6% 7% 4.2% CV (%) 5.3% 6.8% 4.1% 6.6% 7% 4.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.01 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.01 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.01 O.D. 0.001 0.065 0.123 0.224 0.421 0.801 1.387 2.01

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.