Mouse PGLYRP1/Pgrps ELISA Kit PicoKine®

Peptidoglycan recognition protein 1, also known as TAG7, is a protein that in humans is encoded by the PGLYRP1 gene. The PGLYRP1 gene is highly expressed in bone marrow, circulating Polymorphonuclear leukocytes (PMLs), and in the corneal epithelium. The PGLYRP1 protein is primarily found in the granules of PMLs. The PGLYRP1 protein plays an important role in the innate immune response. It is bactericidal against gram-positive bacteria such as S. aureus, S. epidermidis, and L. monocytogenes and generally has proinflammatory effects, inducing TNF-a and IFN-g in many tissues. PGLYRP-1 is also known to form a cytotoxic complex with HSP-70, suggesting it may also have a role in anti-cancer defense. As a pathogen recognition protein with antimicrobial properties, PGLYRP-1 is suspected to play an important role in maintaining the gut microbial flora.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 31 32 32 32 1.08 3.3% Sample 2 223 229 202 199 213 12.96 6% Sample 3 498 462 442 494 474 23.15 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 31 32 32 32 1.08 3.3% Sample 1 34 31 32 32 32 1.08 3.3% Sample 2 223 229 202 199 213 12.96 6% Sample 2 223 229 202 199 213 12.96 6% Sample 3 498 462 442 494 474 23.15 4.8% Sample 3 498 462 442 494 474 23.15 4.8%

Strongly expressed in spleen and lung. Also detected in brain and thymus. In the lung, expressed in the intraalveolar space, in the brain, expressed in the Purkinje cells of the cerebellum and in certain layers of neurons in the hippocampus. Also detected in cells filling the space within the intestinal villus.

Apoptosis, Associated Proteins, Cancer, Cell Biology, Cell Death, Cytokines, Defensin, Human Protein, Immunology, Innate Immunity, Microbiology, Neurology Process, Neuroscience, Protein, Receptors

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 498 37 211 460 Standard deviation 1.87 16.05 27.39 2.03 16.24 26.68 CV (%) 5.5% 7.2% 5.5% 5.5% 7.7% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 498 37 211 460 Standard deviation 1.87 16.05 27.39 2.03 16.24 26.68 CV (%) 5.5% 7.2% 5.5% 5.5% 7.7% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 223 498 37 211 460 Mean (pg/ml) 34 223 498 37 211 460 Standard deviation 1.87 16.05 27.39 2.03 16.24 26.68 Standard deviation 1.87 16.05 27.39 2.03 16.24 26.68 CV (%) 5.5% 7.2% 5.5% 5.5% 7.7% 5.8% CV (%) 5.5% 7.2% 5.5% 5.5% 7.7% 5.8%

Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram- negative bacteria. Involved in innate immunity. May function in intracellular killing of bacteria. The soluble form triggers apoptosis in vitro.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.103 0.175 0.316 0.584 0.992 1.6 2.167 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.103 0.175 0.316 0.584 0.992 1.6 2.167 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.103 0.175 0.316 0.584 0.992 1.6 2.167 O.D. 0.008 0.103 0.175 0.316 0.584 0.992 1.6 2.167

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.