Mouse Mimecan ELISA Kit PicoKine®

Mouse Mimecan ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ogn in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Osteoglycin (also called mimecan) is a protein that in humans is encoded by the OGN gene. This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR) . The level of expression of this gene has been correlated with enlarged hearts and more specifically left ventricular hypertrophy. Mimecan belongs to a family of small leucine-rich proteoglycans (SLRPs) . The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. The OGN gene was mapped to chromosome 9q22.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 198 185 187 190 4.94 2.6% Sample 2 372 421 383 410 396 19.78 4.9% Sample 3 1477 1456 1506 1663 1525 81.34 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 198 185 187 190 4.94 2.6% Sample 1 190 198 185 187 190 4.94 2.6% Sample 2 372 421 383 410 396 19.78 4.9% Sample 2 372 421 383 410 396 19.78 4.9% Sample 3 1477 1456 1506 1663 1525 81.34 5.3% Sample 3 1477 1456 1506 1663 1525 81.34 5.3%

Synonyms

Mimecan; Osteoglycin; Ogn; Og

Gene Name

Ogn

Gene ID

18295

UniProt

Q62000

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-F298

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1%

Sensitivity

<12 pg/mL

Function

Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Mimecan

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Osteoglycin

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.075 0.142 0.258 0.426 0.761 1.266 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.075 0.142 0.258 0.426 0.761 1.266 1.869 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.075 0.142 0.258 0.426 0.761 1.266 1.869 O.D. 0.004 0.075 0.142 0.258 0.426 0.761 1.266 1.869

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.