Rat CCL7/MCP-3 ELISA Kit PicoKine®

Rat CCL7/MCP-3 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Ccl7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3) . It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 38 42 42 40 1.78 4.4% Sample 2 232 236 232 230 232 2.17 0.9% Sample 3 420 474 437 445 444 19.53 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 38 42 42 40 1.78 4.4% Sample 1 39 38 42 42 40 1.78 4.4% Sample 2 232 236 232 230 232 2.17 0.9% Sample 2 232 236 232 230 232 2.17 0.9% Sample 3 420 474 437 445 444 19.53 4.3% Sample 3 420 474 437 445 444 19.53 4.3%

Synonyms

C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Small-inducible cytokine A7; Ccl7; Mcp3, Scya7

Gene Name

Ccl7

Gene ID

287561

UniProt

Q9QXY8

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-P97

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 232 420 42 226 421 Standard deviation 2.34 10.9 18.48 2.89 11.3 20.2 CV (%) 6% 4.7% 4.4% 6.9% 5% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 232 420 42 226 421 Standard deviation 2.34 10.9 18.48 2.89 11.3 20.2 CV (%) 6% 4.7% 4.4% 6.9% 5% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 232 420 42 226 421 Mean (pg/ml) 39 232 420 42 226 421 Standard deviation 2.34 10.9 18.48 2.89 11.3 20.2 Standard deviation 2.34 10.9 18.48 2.89 11.3 20.2 CV (%) 6% 4.7% 4.4% 6.9% 5% 4.8% CV (%) 6% 4.7% 4.4% 6.9% 5% 4.8%

Sensitivity

<10 pg/mL

Function

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat CCL7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine ligand 7

Subcellular Location

Secreted.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.133 0.241 0.461 0.844 1.398 1.845 1.999 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.133 0.241 0.461 0.844 1.398 1.845 1.999 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.133 0.241 0.461 0.844 1.398 1.845 1.999 O.D. 0.012 0.133 0.241 0.461 0.844 1.398 1.845 1.999

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.