Human PD-L2/B7-DC ELISA Kit PicoKine®

Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 126 127 117 125 5.71 4.5% Sample 2 396 453 452 415 429 24.44 5.6% Sample 3 998 945 1041 1035 1004 38.22 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 126 127 117 125 5.71 4.5% Sample 1 133 126 127 117 125 5.71 4.5% Sample 2 396 453 452 415 429 24.44 5.6% Sample 2 396 453 452 415 429 24.44 5.6% Sample 3 998 945 1041 1035 1004 38.22 3.8% Sample 3 998 945 1041 1035 1004 38.22 3.8%

Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273; PDCD1LG2; B7DC, CD273, PDCD1L2, PDL2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 396 998 133 415 903 Mean (pg/ml) 133 396 998 133 415 903 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 Standard deviation 7.18 28.11 57.318 7.31 37.35 54.18 CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6% CV (%) 5.4% 7.1% 5.8% 5.5% 9% 6%

Involved in the costimulatory signal, essential for T- cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.