Mouse PON1/Paraoxonase 1 ELISA Kit PicoKine®

Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 310 330 311 328 21.85 6.6% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 3 5447 5579 4941 5080 5261 260.23 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 364 310 330 311 328 21.85 6.6% Sample 1 364 310 330 311 328 21.85 6.6% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 2 1139 1282 1324 1250 1248 68.58 5.4% Sample 3 5447 5579 4941 5080 5261 260.23 4.9% Sample 3 5447 5579 4941 5080 5261 260.23 4.9%

Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 364 1139 5447 343 1198 5787 Mean (pg/ml) 364 1139 5447 343 1198 5787 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 Standard deviation 21.47 54.67 403.07 25.38 79.06 468.74 CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1% CV (%) 7.4% 4.8% 7.4% 7.4% 6.6% 8.1%

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.467 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.