Human TRAILR3/TNFRSF10C ELISA Kit PicoKine®

TRAILR3/TNFRSF10C is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. TRAILR3 gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 163 147 142 151 7.88 5.2% Sample 2 584 548 550 549 557 15.17 2.7% Sample 3 1628 1534 1759 1601 1630 81.7 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 163 147 142 151 7.88 5.2% Sample 1 154 163 147 142 151 7.88 5.2% Sample 2 584 548 550 549 557 15.17 2.7% Sample 2 584 548 550 549 557 15.17 2.7% Sample 3 1628 1534 1759 1601 1630 81.7 5% Sample 3 1628 1534 1759 1601 1630 81.7 5%

Tumor necrosis factor receptor superfamily member 10C; Antagonist decoy receptor for TRAIL/Apo-2L; Decoy TRAIL receptor without death domain; Decoy receptor 1; DcR1; Lymphocyte inhibitor of TRAIL; TNF-related apoptosis-inducing ligand receptor 3; TRAIL receptor 3; TRAIL-R3; TRAIL receptor without an intracellular domain; CD263; TNFRSF10C; DCR1, LIT, TRAILR3, TRID; UNQ321/PRO366

Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 584 1628 145 568 1473 Standard deviation 8.31 40.88 109.07 9.42 46 103.11 CV (%) 5.4% 7% 6.7% 6.5% 8.1% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 584 1628 145 568 1473 Standard deviation 8.31 40.88 109.07 9.42 46 103.11 CV (%) 5.4% 7% 6.7% 6.5% 8.1% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 584 1628 145 568 1473 Mean (pg/ml) 154 584 1628 145 568 1473 Standard deviation 8.31 40.88 109.07 9.42 46 103.11 Standard deviation 8.31 40.88 109.07 9.42 46 103.11 CV (%) 5.4% 7% 6.7% 6.5% 8.1% 7% CV (%) 5.4% 7% 6.7% 6.5% 8.1% 7%

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.077 0.141 0.231 0.419 0.797 1.263 1.72 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.077 0.141 0.231 0.419 0.797 1.263 1.72 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.01 0.077 0.141 0.231 0.419 0.797 1.263 1.72 O.D. 0.01 0.077 0.141 0.231 0.419 0.797 1.263 1.72

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.