Human BMP-9 ELISA Kit PicoKine®

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 31 31 33 2 6% Sample 2 186 184 182 187 184 1.92 1% Sample 3 405 398 405 389 399 6.57 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 31 31 33 2 6% Sample 1 35 35 31 31 33 2 6% Sample 2 186 184 182 187 184 1.92 1% Sample 2 186 184 182 187 184 1.92 1% Sample 3 405 398 405 389 399 6.57 1.6% Sample 3 405 398 405 389 399 6.57 1.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5%

Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.