Human BMP-9 ELISA Kit PicoKine®

Human BMP-9 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GDF2 in bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 31 31 33 2 6% Sample 2 186 184 182 187 184 1.92 1% Sample 3 405 398 405 389 399 6.57 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 31 31 33 2 6% Sample 1 35 35 31 31 33 2 6% Sample 2 186 184 182 187 184 1.92 1% Sample 2 186 184 182 187 184 1.92 1% Sample 3 405 398 405 389 399 6.57 1.6% Sample 3 405 398 405 389 399 6.57 1.6%

Synonyms

Growth/differentiation factor 2; GDF-2; Bone morphogenetic protein 9; BMP-9; GDF2; BMP9

Gene Name

GDF2

Gene ID

2658

UniProt

Q9UK05

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S320-R429

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum plasma (heparin, EDTA) and urine.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 186 405 34 178 376 Mean (pg/ml) 35 186 405 34 178 376 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 Standard deviation 2.06 9.3 19.44 2.48 11.92 24.44 CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5% CV (%) 4.8%% 5% 4.8% 7.3% 6.7% 6.5%

Sensitivity

<10 pg/mL

Function

Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human BMP-9

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth differentiation factor 2

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388 O.D. 0.051 0.116 0.184 0.322 0.612 1.103 1.738 2.388

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.