Rat BMP-9 ELISA Kit PicoKine®

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 36 36 38 36 0.86 2.3% Sample 2 187 182 199 172 185 9.72 5.2% Sample 3 414 464 457 446 445 19.14 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 36 36 38 36 0.86 2.3% Sample 1 36 36 36 38 36 0.86 2.3% Sample 2 187 182 199 172 185 9.72 5.2% Sample 2 187 182 199 172 185 9.72 5.2% Sample 3 414 464 457 446 445 19.14 4.3% Sample 3 414 464 457 446 445 19.14 4.3%

Growth differentiation factor 2 ; Similar to Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) ; Gdf2 ; LOC290921 ; rCG_42312

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.