Rat BMP-9 ELISA Kit PicoKine®

Rat BMP-9 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Gdf2 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP) -9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 36 36 38 36 0.86 2.3% Sample 2 187 182 199 172 185 9.72 5.2% Sample 3 414 464 457 446 445 19.14 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 36 36 38 36 0.86 2.3% Sample 1 36 36 36 38 36 0.86 2.3% Sample 2 187 182 199 172 185 9.72 5.2% Sample 2 187 182 199 172 185 9.72 5.2% Sample 3 414 464 457 446 445 19.14 4.3% Sample 3 414 464 457 446 445 19.14 4.3%

Synonyms

Growth differentiation factor 2 ; Similar to Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) ; Gdf2 ; LOC290921 ; rCG_42312

Gene Name

Gdf2

Gene ID

290921

UniProt

M0RB87

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: S320-R430

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 187 414 35 192 435 Mean (pg/ml) 36 187 414 35 192 435 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 Standard deviation 2.12 11.78 23.59 2.69 13.63 27.16 CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9% CV (%) 5.7% 7.1% 5.7% 7.7% 7.1% 5.9%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

47978 MW

Specificity

Natural and recombinant rat BMP-9

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Growth differentiation factor 2

Subcellular Location

Secreted .

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077 O.D. 0.067 0.13 0.168 0.25 0.427 0.772 1.317 2.077

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.